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        Signature
      
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        /s/ Shannon Atchison, Attorney in Fact
      
      
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        Stock symbol
      
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        NSTG
      
      
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        Transactions as of
      
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        May 6, 2024
      
      
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        Transactions value $
      
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        $0
      
      
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        Form type
      
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        4
      
      
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        Date filed
      
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        5/8/2024, 08:48 PM
      
 
     
   
 
  
    Transactions Table
  
  
    
      
        
          | Type | Sym | Class | Transaction | Value $ | Shares | Change % | * Price $ | Shares After | Date | Ownership | Footnotes | 
      
      
          
            | transaction | NSTG | Common Stock | Options Exercise | $0 | +200K |  | $0.00 | 200K | May 6, 2024 | Direct | F1 | 
      
    
   
  
    Derivative Securities (e.g., puts, calls, warrants, options, convertible securities)
  
  
    
      
        
          | Type | Sym | Class | Transaction | Value $ | Shares | Change % | * Price $ | Shares After | Date | Underlying Class | Amount | Exercise Price | Ownership | Footnotes | 
      
      
          
            | transaction | NSTG | Restricted Stock Units | Options Exercise | $0 | -200K | -100% | $0.00* | 0 | May 6, 2024 | Common Stock | 200K |  | Direct | F2, F3 | 
      
    
   
  
    * An asterisk sign (*) next to the price indicates that the price is likely invalid.
  
  Jonathan Todd Garland is no longer subject to Section 16 filing requirements. Form 4 or Form 5 obligations may continue.
  
    Explanation of Responses: