Paul B. Manning - May 30, 2025 Form 4 Insider Report for Taysha Gene Therapies, Inc. (TSHA)

Role: 10%+ Owner
Signature: /s/ Kamran Alam, Attorney-in-Fact
Stock symbol: TSHA
Transactions as of: May 30, 2025
Transactions value $: $2,062,500
Form type: 4
Date filed: 6/3/2025, 09:38 PM
Previous filing: Dec 18, 2024
Next filing: Jun 20, 2025
SEC: View on sec.gov

Reporting Owners (1)

Name	Relationship	Address	Signature	Signature date	CIK
Manning Paul B	10%+ Owner	200 GARRETT STREET, SUITE S, CHARLOTTESVILLE	/s/ Kamran Alam, Attorney-in-Fact	2025-06-03	0001494695

Transactions Table

Type	Sym	Class	Transaction	Value $	Shares	Change %	* Price $	Shares After	Date	Ownership	Footnotes
transaction	TSHA	Common Stock	Purchase	$2.06M	+750K	+35.86%	$2.75	2.84M	May 30, 2025	See footnote	F1
holding	TSHA	Common Stock						14.7M	May 30, 2025	See footnote	F2, F3
holding	TSHA	Common Stock						22K	May 30, 2025	See footnote	F4
holding	TSHA	Common Stock						4.01M	May 30, 2025	See footnote	F2, F5
holding	TSHA	Common Stock						4M	May 30, 2025	See footnote	F2, F6

* An asterisk sign (*) next to the price indicates that the price is likely invalid.

Paul B. Manning is no longer subject to Section 16 filing requirements. Form 4 or Form 5 obligations may continue.

Explanation of Responses:

Id	Content
F1	The shares are held directly by BKB Growth Investments, LLC ("BKB"). The Reporting Person is a co-manager of Tiger Lily Capital, LLC, the manager of BKB, and has shared voting and investment power with respect to the shares held by BKB.
F2	The change in the total shares held prior to the transactions reported herein reflects a change in form of ownership and, therefore, was not required to be reported pursuant to Section 16.
F3	The shares are held directly by The Paul B. Manning Revocable Trust dated May 10, 2000 (the "Revocable Trust"). The Reporting Person is the trustee of the Revocable Trust and has sole voting and investment power with respect to the shares held by the Revocable Trust.
F4	The shares are held directly by BKB G2 Investments, LLC ("BKB G2"). The Reporting Person is a co-manager of Tiger Lily Capital, LLC, the manager of BKB G2, and has shared voting and investment power with respect to the shares held by BKB G2.
F5	The shares are held directly by The PBM 2023 Grantor Retained Annuity Trust (the "2023 Annuity Trust"). The Reporting Person is the trustee of the 2023 Annuity Trust and has sole voting and investment power with respect to the shares held by the 2023 Annuity Trust.
F6	The shares are held directly by The PBM 2025 Grantor Retained Annuity Trust (the "2025 Annuity Trust"). The Reporting Person is the trustee of the 2025 Annuity Trust and has sole voting and investment power with respect to the shares held by the 2025 Annuity Trust.