| Name | Relationship | Address | Signature | Signature date | CIK |
|---|---|---|---|---|---|
| Burton Paul Frederick | Director | C/O CARDIO DIAGNOSTICS HOLDINGS INC., 311 W. SUPERIOR ST. SUITE 444, CHICAGO | /s/ Elisa Luqman as attorney-in-fact for Paul Burton | 2025-10-03 | 0002006956 |
| Type | Sym | Class | Transaction | Value $ | Shares | Change % | * Price $ | Shares After | Date | Underlying Class | Amount | Exercise Price | Ownership | Footnotes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| transaction | CDIO | Stock Options (Right to Buy) | Award | $6.25K | +1.56K | +45.93% | $4.01 | 4.95K | Sep 30, 2025 | Common Stock | 1.56K | $4.01 | Direct |